Result
2014/9/26 5:03:15 
239 
2Two Col2.3Bcl-2 (tg/+) founder lines were established and appeared normal except that they were smaller than their nontransgenic wildtype (+/+) littermates at 1, 2, and 6 months of age, with the greatest differences at 2 months. Immunohistochemistry showed hBcl-2 in osteoblasts at the growth plate and cortical surfaces. Nontransgenic littermates were negative. Western blots revealed hBcl-2 only in type I collagen-expressing tissues. Histomorphometry of 2-month-old mice showed a significant decrease in tg/+ calvaria width with no significant differences in femoral trabecular area or cortical width compared with +/+. However, tg/+ males had significantly more trabecular bone than tg/+ females. Female +/+ mice showed increased bone turnover with elevated osteoblast and osteoclast parameters compared with +/+ males. Col2.3Bcl-2 mice did not show such significant differences between sexes. Male tg/+ mice had a 76.5 +/- 1.5% increase in ObS/BS with no significant differences in bone formation rate (BFR) or mineral apposition rate (MAR) compared with male +/+ mice. Transgenic females had a significant 48.4 +/- 0.1% and 20.1 +/- 5.8% decrease in BFR and MAR, respectively, compared with +/+ females. Osteoclast and osteocyte parameters were unchanged. By 6 months, femurs from female and male +/+ mice had lost a significant amount of their percent of trabecular bone compared with 2-month-old mice. There was little to no change in femoral bone in the tg/+ mice with age. Ex vivo cultures of osteoblasts from +/+ and Col2.3Bcl-2 mice showed a decrease in mineralization, no effect on proliferation, and an inhibition of glucocorticoid-induced apoptosis in Col2.3Bcl-2 cultures. Estrogen was shown to increase mouse Bcl-2 transcript levels in osteoblast cultures of wildtype mice, supporting a role for Bcl-2 in the sex-related differences in bone phenotype regulated by estrogen. Therefore, Bcl-2 differentially affected bone phenotype in male and female transgenic mice, altered bone cell activity associated with sex-related differences, and decreased bone formation, suggesting that apoptosis is necessary for mineralization. In addition, Bcl-2 targeted to mature osteoblasts seemed to delay bone development, producing a smaller transgenic mouse compared with wildtype littermates. These studies suggest that expression of Bcl-2 in osteoblasts is important in regulating bone mass in development and in the normal aging process of bone.
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热评图文
用料
鸡翅10只可乐半罐（一罐330毫升）酱油2勺老抽1小勺八角1个桂皮1小块姜 葱各适量米酒1勺(因本人不喜欢料酒味，平时都用米酒去腥）
可乐鸡翅（家常版）的做法步骤
步骤 1
鸡翅洗干净 两面划刀 沥干水份 加一勺酱油 适量姜丝 一小勺米酒 腌制一个小时以上
步骤 2
腌制好的鸡翅冷水下锅 加入葱 姜焯水 煮出浮沫 撇去浮沫 捞出 温水洗净 沥干..
虎皮青椒
用料
青椒5个蒜末2瓣生抽2勺老抽0.5勺醋1勺白糖1勺淀粉1勺油耗油
虎皮青椒的做法步骤
步骤 1
做法： ①调酱汁：2勺生抽+半勺老抽+1勺陈醋+1勺白糖+一勺淀粉+少许清水搅拌均匀。 喜欢耗油的可以再+1勺耗油
步骤 2
②青椒洗净去蒂切段，电炒锅里适量油，放青椒煎出虎皮，放入蒜末，倒入料汁焖煮收汁即可~
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